ANTUREC related publications
This lists different resources about ANTUREC and it's science. This includes preclinical studies, clinical studies and information on the TRABTRAP study.
Manufacturer´s Licence - GMP production
The University Hospital of Muenster, Germany (UKM) - together with ANTUREC - holds a GMP manufacturing licence for the IMP tTF-NGR used for multicenter clinical studies
(Manufacturer´s authorisation number DE_NW_05_MIA_2021_0009/24.05.03-034).
Related Publications
This list includes preclinical studies, clinical studies, information on "Good Manufacturing Practice" (GMP) guidelines and information on the tTF-NGR working group of the university hospital Muenster.
Preclinical Studies
- Kessler, T.; et al. Inhibition of tumor growth by RGD peptide-directed delivery of truncated tissue factor to the tumor vasculature. Clin Cancer Res. 2005, 11, 6317-6324. doi:10.1158/1078-0432.CCR-05-0389.
- Persigehl, T.; et al. Antiangiogenic tumor treatment: early noninvasive monitoring with USPIO-enhanced MR imaging in mice. Radiology. 2007, 244, 449–456. doi: 10.1148/radiol.2442060371.
- Persigehl, T.; et al. Prediction of antiangiogenic treatment efficacy by iron oxide enhanced parametric magnetic resonance imaging. Investig Radiol. 2007, 42, 791–796. doi: 10.1097/RLI.0b013e3180d5cbd9.
- Kessler, T.; et al. Generation of fusion proteins for selective occlusion of tumor vessels. Curr Drug Discov Technol. 2008, 5, 1–8. DOI: 10.2174/157016308783769487.
- Bieker, R.; et al. Infarction of tumor vessels by NGR-peptide directed targeting of tissue factor. Experimental results and first-in-man experience. Blood. 2009, 113, 5019–5027. DOI: 10.1182/blood-2008-04-150318.
- Schwöppe, C.; et al. Tissue-factor fusion proteins induce occlusion of tumor vessels. Thromb Res. 2010, 125, S143–S150. DOI: 10.1016/S0049-3848(10)70033-5.
- Dreischalück, J.; et al. Vascular infarction by subcutaneous application of tissue factor targeted to tumor vessels with NGR-peptides: activity and toxicity profile. Int J Oncol. 2010, 37, 1389–1397. DOI: 10.3892/ijo_00000790.
- Von Maltzahn, G.; et al. Nanoparticles that communicate in vivo to amplify tumour targeting. Nat Mater. 2011, 10, 545–552. DOI: 10.1038/nmat3049.
- Schwöppe, C.; et al. Using native gel electrophoresis or isoelectric focusing as experimental “clock” for the (iso)aspartate formation of tTF-NGR fusion proteins. Biomacromol. Mass Spectrom. 2012, 2, 285–293.
- Schwöppe, C.; et al. Anticancer therapy by tumor vessel infarction with polyethylene glycol conjugated retargeted tissue factor. J Med Chem. 2013, 56, 2337–2347. DOI: 10.1021/jm301669z.
- Persigehl, T.; et al. Non-invasive monitoring of tumor-vessel infarction by retargeted truncated tissue factor tTF-NGR using multi-modal imaging. Angiogenesis. 2014, 17, 235–246. doi: 10.1007/s10456-013-9391-4.
- Brand, C.; et al. Low-energy ultrasound treatment improves regional tumor vessel infarction by retargeted tissue factor. J Ultrasound Med. 2015, 34, 1227–1236. DOI: 10.7863/ultra.34.7.1227.
- Brand, C.; et al. Tumor growth inhibition via occlusion of tumor vasculature induced by N-terminally PEGylated retargeted tissue factor tTF-NGR. Mol Pharm. 2015, 12, 3749–3758. DOI: 10.1021/acs.molpharmaceut.5b00508.
- Brand, C.; et al. NG2 proteoglycan as a pericyte target for anticancer therapy by tumor vessel infarction with retargeted tissue factor. Oncotarget. 2016, 7, 6774-6789. doi: 10.18632/oncotarget.12559.
- Stucke-Ring, J.; et al. Combinatorial effects of doxorubicin and retargeted tissue factor by intratumoral entrapment of doxorubicin and proapoptotic increase of tumor vascular infarction. Oncotarget. 2016, 7, 82458–82472. DOI: 10.18632/oncotarget.12559.
- Schmidt, L.H.; et al. Potential therapeutic impact of CD13 expression in non-small cell lung cancer. PLoS ONE. 2017, 12, e0177146. DOI: 10.1371/journal.pone.0177146.
- Schmidt, L.H.; et al. CD13 as target for tissue factor induced tumor vascular infarction in small cell lung cancer. Lung Cancer. 2017, 113, 121–127. DOI: 10.1016/j.lungcan.2017.09.013.
- Kessler, T.; et al. Aminopeptidase N (CD13): Expression, prognostic impact, and use as therapeutic target for tissue factor induced tumor vascular infarction in soft tissue sarcoma. Transl Oncol. 2018, 11, 1271–1282. DOI: 10.1016/j.tranon.2018.08.004.
- Höink, A.; et al. Gadofosveset-enhanced MRI as simple surrogate parameter for real-time evaluation of the initial tumour vessel infarction by retargeted tissue factor tTF-NGR. Oncol Lett. 2019, 17, 270–280. DOI: 10.3892/ol.2018.9638.
- Brand, C.; et al. Radiation synergizes with antitumor activity of CD13-targeted tissue factor in a HT1080 xenograft model of human soft tissue sarcoma. PLoS ONE. 2020, 15, e0229271. DOI: 10.1371/journal.pone.0229271
Clinical Studies
- Gerwing, M.; et al. The beginning of the end for conventional RECIST—novel therapies require novel imaging approaches. Nat Rev Clin Oncol. 2019, 16, 442–458. DOI: 10.1038/s41571-019-0169-5.
- Schliemann, C.; et al. First-in-class CD13-targeted tissue factor tTF-NGR in patients with recurrent or refractory malignant tumors: results of a phase I dose-escalation study. Cancers. 2020, 12, 1488. DOI: 10.3390/cancers12061488.
- Berdel, W.E.; et al. Animal safety, toxicology, and pharmacokinetic studies according to the ICH S9 guideline for a novel fusion protein tTF-NGR targeting procoagulatory activity into tumor vasculature: are results predictive for humans? Cancers. 2020, 12, 3536. DOI: 10.3390/cancers12123536.
- Gerwing M.; et al. Multiparametric Magnetic Resonance Imaging for Immediate Target Hit Assessment of CD13-Targeted Tissue Factor tTF-NGR in Advanced Malignant Disease. Cancers 2021, 13, 5880, DOI: 10.3390/cancers 1323588.
- Hoffmann, E; et al. Multiparametric MRI for characterization oft he tumour microenvironment. Nat Rev Clin Oncol. 2024, 21, 428-448. DOI: 10.1038/s41571-024-00891-1.
- Heßling, K; et al. Trabectedin versus trabectedin plus CD13-targeted tissue factor tTF-NGR in patients with advanced relapsed or refractory soft tissue sarcoma: translational data and clinical dose-finding cohort from the TRABTRAP study. Scientific Reports. 2026, 16, 7389. DOI: 10.1038/s41598‑026‑40362‑4.






